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McCauley et al. 2018 . Predicted fluorescence protein expression patterns from the basal cell reporter are shown in color. (C) Representative image demonstrating GFP and tdTomato fluorescence expression in BU3-NGPT on day 15 (upper panels) or day 29 (lower panels) of differentiation. Scale bar = 100 μm Representative BU3-NGPT culture in one Matrigel droplet on day 29 of differentiation (10× magnification, 6 × 6 tile scan). " width="100%" height="100%">
Journal: STAR Protocols
Article Title: Differentiation of human pluripotent stem cells into functional airway basal stem cells
doi: 10.1016/j.xpro.2021.100683
Figure Lengend Snippet: Direct differentiation of hPSC-derived airway epithelial organoids from basal cell reporter iPSCs, BU3-NGPT iPSCs (A) Genomic structure of the basal cell reporter in BU3-NGPT iPSCs. eGFP gene is integrated in both alleles of NKX2-1 and tdTomato gene is integrated in one allele of TP63 . (B) Schematic of the airway-directed differentiation protocol detailed in
Article Snippet:
Techniques: Derivative Assay, Fluorescence, Expressing
Journal: STAR Protocols
Article Title: Differentiation of human pluripotent stem cells into functional airway basal stem cells
doi: 10.1016/j.xpro.2021.100683
Figure Lengend Snippet: Examination and isolation of iBCs (A) Representative image of organoid on day 40 of differentiation showing GFP and tdTomato expression (left panel) and its morphology (middle panel). Representative BU3-NGPT culture in one Matrigel droplet on day 40 of differentiation (Right panel, 10× magnification, 7 × 7 tile scan). (B) Representative expression pattern of NGFR and EpCAM (left panel) or GFP and tdTomato (middle panel) in single live cells of BU3-NGPT on day 40. iBCs are gated first for NKX2-1 GFP+ and TP63 tdTomato+ population in bold line square (middle panel) and then sorted for NGFR + EpCAM + population in bold line square (right panel). (C) Representative expression pattern of NGFR and EpCAM (left panel) in single live cells of non-reporter iPSC on day 44. The suggested gating for NGFR + EpCAM + iBC population is shown with bold line square.
Article Snippet:
Techniques: Isolation, Expressing
Journal: STAR Protocols
Article Title: Differentiation of human pluripotent stem cells into functional airway basal stem cells
doi: 10.1016/j.xpro.2021.100683
Figure Lengend Snippet:
Article Snippet:
Techniques: Control, Recombinant, Software, Membrane, Gentle, Modification, Blocking Assay, Plasmid Preparation, Microscopy